Features and Benefits

Compatible with high-throughput handling systems.

General description

Ammonia (NH₃) or the ammonium ion (NH₄ ⁺) is found in the atmosphere, rainwater, soil, seawater, and volcanic areas. It is widely used as fertilizer, being an important source of nitrogen for living systems. It also plays a role in animal physiology. Ammonia is produced by amino acid metabolism and is converted to urea in the human liver through the urea cycle. Ammonia acts as a precursor for various other nitrogen compounds. It is a colorless gas with pungent odor and can be easily liquefied. It is also used as a feed supplement in livestock industry.

Principle

The Ammonia Assay Kit provides a simple and high throughput adaptable assay for quantitative determination of ammonia/ammonium ion concentration in biological samples, such as urine, and environmental samples. This assay is based on the o-phthalaldehyde method in which the reagent reacts with ammonia/ammonium ion producing a fluorometric result ( ex = 360/ em = 450 nm), proportional to the ammonia concentration in the sample. The kit has a linear detection range of 0.012–1 mM ammonia in a 96 well format.

Suitability

Suitable for the quantitative determination of ammonia/ammonium concentration in biological samples, such as urine, and environmental samples